Whether High Temperature in Incubator Can Eliminate Vernalization of Low-temperature Seedlings

After 21st, 28th and 35th of the vernalization of winter wheat shoots, the heading potential was obtained. If the light incubator was treated with light for 5 days and 35°C, the heading ability was lost after sowing. If the winter wheat is subjected to an ultra-vernal vernalization for a period of 70 days, the dewaxing does not work, and specific proteins are still detected in the young shoots. Vernalization gene expression is generally only released at higher temperatures (33±2° C.) and longer (5 d). If the vernalization process is completed (period vernalization), it cannot be released by high temperatures. Detection of proteins in reverted shoots revealed that the specific proteins associated with vernalization disappeared. This shows that vernalization-induced flowering potential has been lifted by declarification.

During the process of artificial low-temperature treatment, the effects of light intensity and illumination time on the vernalization of rape and the suitable illumination indicators required for vernalization have not been systematically reported. Light incubator research on the impact of light time on the growth period, Sun Chaocai et al reported that extending the daily light time can make rape mature ahead of time, can shorten the generation time. The issues such as the appropriate light intensity and time during the artificial vernalization of rapeseed and its interaction with temperature need to be further studied.

Vernalization temperatures are low and vernalization time can be shortened. If rapeseed microspores are planted in the field to avoid the high temperatures after mid-July, they need to be shortened as much as possible. However, in the summer of Wuhan, the temperature of equipment such as light incubators is difficult to stabilize below 6°C. Therefore, we can try to hypocotylize the cotyledonary stage embryoid bodies or adventitious buds regenerated from the embryoid bodies, and then transfer the vernalized embryoid bodies and buds to a new culture medium and culture them at 25°C to become a viable transplanted seedling. To shorten the time of early seedlings.

The plantlets in the light incubator can be treated with low temperatures during spring flowering. If the plants are moved outdoors outside the middle of May for sufficient light, they can produce normal germinating seeds. The above distribution can be used to construct genetic research and related research of double haploid breeding. This also shows that the light incubator seedlings must be environmentally transferred before mid-May to avoid the summer heat environment. The moving environment should be as cool and airy as possible to reduce the temperature.

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