Study on Non-in vitro Determination of Leaf Area of ​​Buckwheat

Study on Non-in vitro Determination of Leaf Area of ​​Buckwheat

The buckwheat leaves are thin, easy to curl and easily tear, and the non-in vitro rapid assay method is easy to operate without damaging the leaves and the results are accurate.

Materials: Leaves of different varieties of buckwheat varieties such as tartary buckwheat, sweet buckwheat, golden buckwheat, wild winged pheasant, small wild pheasant and hard-bred variegated nymph.

Equipment: Leaf area meter, coordinate paper, ruler, pencil, counter, and small scissors.

The buckwheat leaves are divided into two types. The main shapes of the buckwheat leaves are oval, heart-shaped, triangular and cocoon-shaped. The main shapes of the two types are approximately lancet-shaped heart-shaped and arrow-shaped.

Take 20 to 30 pieces of two types of leaves, spread them on white paper and transparent grid paper, draw a leaf shape, and use a small pair of scissors to cut out the leaf shape on the white paper and measure it with a live leaf area meter. The area is determined by a counter at the same time, and the area of ​​the leaf shape on the clear grid paper is determined. The real area of ​​the leaves is S1, S2,..., Sn respectively.

Take 20-30 pieces of two types of leaves, spread them on a white paper, and use a pencil to hit a point on the white paper at the tip of the blade, and then use a pencil to hit a point on the white paper on the two sides of the blade, that is, three points. A triangle can be determined and the area of ​​the triangle is calculated as ΔS1, ΔS2,... ΔSn.

Calculate the conversion factor K. K=S1+S2+...+Sn/(ΔS1+ΔS2+...+ΔSn)

Analysis of results: By calculating the conversion coefficient, for a class of leaves, the K value is 1.1, the second class leaves, and the K value is 0.9. Therefore, the calculation of the area of ​​the buckwheat leaf is converted into the area of ​​the triangle by conversion and the correlation coefficient is multiplied. Calculate the lancet-shaped heart-shaped and arrow-shaped leaf area, S = 0.9 △ S to calculate the remaining shape of the leaf area, and the above two types of leaf shape easier to distinguish, it can be used to conduct non-isolated rapid determination of buckwheat leaf area.