Study on Breeding of Spores of Sclerotinia fulvum in Artificial Climate Incubator
The mango anthracnose caused by Colletotrichum anthracis is one of the most important diseases that seriously affect the yield and quality of mango. The expansion of leaf lesions in leaf spot disease is the result of the joint action of host, pathogen, and environment. It reflects the severity of the disease. The rapid expansion of the lesion reflects the severity of the disease, and the slow disease expansion reflects the disease. Little harm. There are many factors that affect the spread of lesions, including variety disease resistance, effective accumulated temperature, temperature, relative humidity, mineral elements and soil fertility and other factors. The influence of different factors on the spread of different lesions has been studied. Disease outbreaks in large areas are inseparable from the appropriate disease environment. Fully studying the influence of disease factors on the development of diseases is a prerequisite for scientific management of plant diseases. There are few studies on the influencing factors of the expansion of mango anthracnose lesions. The effects of conidial inoculum concentration, mango disease resistance, temperature, relative humidity, and light on the spread of mango anthracnose lesions were studied by in vitro leaf inoculation, aiming at revealing the main factors affecting the spread of mango anthracnose lesions. Factors to understand the occurrence and development of mango anthracnose. Artificial climate incubators play an important role in the research process.
Five light green leaves of mango with the same size were selected, and three leaves of the leaf between the lateral veins and lateral veins were selected on the back of the leaves. The leaves were inoculated with a beam needle and inoculated with a suspension of 10, L, L, with a concentration of 4x105. /mL. Treated with fresh water as a control. Placed in 280C, 100% humidity moisturizing, dark conditions artificial climate incubator cultured, using the cross-measuring method to measure lesion diameter. Effect of pathogenic conidial spore concentration on lesion spread Using the hemocytometer counting method to configure the test conidial suspension into 4x10', 4x106, 4x105, 4x104, 4x10;, 4x102//mL 6 concentrations in total gradient. In vitro leaf inoculation was used to measure lesion diameter after 48, 72, and 96 hours of inoculation. Each treatment was repeated 15 times. All statistical analyses were performed using the DPS data processing system for Duncan multiple recombination and range analysis. Set full illumination (fluorescent lamp, 15W), 12h dark + 12h light (fluorescent lamp, 15W) and total darkness for a total of 3 lighting conditions. Using in vitro leaf inoculation method, conidial concentration 4x105/mL. The leaves were placed under different light conditions and cultured in an artificial climate incubator at 28°C. The vertical distance between the leaves and the light source was 30, 100% humidity, and the diameter of the lesions was measured after 96, 120, and 144 hours. Statistical analysis was performed.
The comprehensive analysis of the effect of spore concentration on the spread of lesions using an artificial climatic incubator showed that after the successful infestation of the conidia at different concentrations and the manifestation of the disease, the lesions had a uniform rate of expansion. The mean expansion rate of the lesions was 48 to 72 hours. 4.4mm/24h, and the average spread rate of lesions at 7296h was 6.0mm/24h, which indicates that the increase of the lesion's expansion speed has an increasing trend over time. 4xlOZ/mL inoculation point inoculation amount 10}, L, so the number of inoculated conidiophores is about 4, reflecting the high virulence characteristics of the bacteria. Field surveys have found that there is a large number of bacterial sources in deciduous, dry branches, leaves, and branches of winter fruits before flowering and extraction of young leaves of mangoes. Therefore, the number of inoculums is one of the secondary factors that influence the spread of lesions.
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