Determination of Chlorophyll Content in Peanut Using Canopy Chlorophyll Meter
When determining the chlorophyll content of peanuts, it is only representative of the leaves at which the leaves are selected. In the past, there has been no specific report on this. In order to understand the changes in the chlorophyll content in different leaf positions of peanuts, the following tests were specially carried out so as to select a suitable leaf position in the sampling process in the future and provide the basis. In addition, the method of extracting chlorophyll, according to the current information describes the use of multi-grinding method, that is, with a mortar and pestle, a small amount of organic fertilizer (acetone) and a small amount of sodium carbonate, quartz sand jointly grinding, filtration, washing, constant volume. However, the grinding method has a lot of workmanship, especially when there are many sample woods to be measured, the operation is slightly indifferent, and errors are likely to occur, and the acetone is expensive and the cost is high. In recent years, ethanol has been used as a solvent (two times) for leaching. The operation is simple, and 95 intestines are more easily obtained. However, there is no difference in the amount of chlorophyll extracted from fresh leaves of the same peanut by the extraction method compared with the method of grinding. Further tests are necessary to correct the data we have obtained. The canopy chlorophyll analyzer can effectively determine the content of chlorophyll in peanuts. The determination.
For determination of chlorophyll content in different leaf positions of peanuts, 8 to 10 plants were randomly selected from each field in Daejeon. Each plant was cut from the parietal lobe (first leaf) of the main stem, and then cut in turn to the 4th to 5th leaf. For each plant as a sample, the leaves of each leaf position are treated as one. When cutting the leaves, pay attention to avoid the main vein, weigh 0.5g of fresh sample each time, and finally use a 25ml volumetric flask (or a plug). Pipe) constant volume. The extract was colorimetrically measured with a 72 or 721 spectrophotometer. The chlorophyll a and b have the same specific absorption coefficient at 652 nm, and their optical densities are measured at this wavelength to calculate the total chlorophyll content, or the optical densities thereof are measured using the two absorption peaks at both 663 nm and 645 nm wavelengths. The chlorophyll a or b content and total amount were calculated separately.
The results of canopy chlorophyll analyzer found that peanuts provided nitrogen due to the nitrogen-fixing effect of rhizobia, especially after blooming, nitrogen fixation was strong. Therefore, in the middle and later stages (19 leaves), if the top 5 leaf is well protected, it can still maintain its higher chlorophyll content, which is beneficial to promote the photosynthesis of the peanut and the development of the node. But taking into account the serious damage caused by rusting flowers in the south, especially spring peanuts, rapid spread after the onset of disease, the lower leaves are easy to first die or covered with piles, such as the fixed fifth leaves, it is often difficult to obtain complete and ideal leaves . Therefore, it is advisable to fix the top third leaf for the sampling leaf.
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